In the recent years, the environment friendly lubricants have been using worldwide. Vegetable oils-based lubricants are the best substitute of the mineral and synthetic lubricant. In view of this present study concerns on tribological investigation of bitter almonds oil containing TiO 2 nanoparticles and Triton X-100 as surfactant. Four different volume fractions of nanoparticles (0.01, 0.02, 0.03, and 0.04) used for nano lubricant formulation and the ratio of TiO 2 nanoparticles and Triton X-100 is 2: 1. The tribological investigation of formulated Bitte almond oil-based nano lubricant on Four ball tribotester using ASTM 4172B. Bitter almonds oils containing 0.02 vol. fraction nanoparticles showed minimum average wear scar diameter and coefficient of friction.